Epitope mapping can reveal how a mAb exerts its functional effects - for instance, by blocking the binding of a ligand or by trapping a protein in a non-functional state.

Information about the binding epitope of an antibody can provide important mechanistic insights and indicate for what applications an antibody might be useful. Therefore, a variety of epitope mapping …

Jul 2, 2025 · The term “epitope mapping” can be defined as the experimental process of locating the epitope of a specific antibody on the antigen surface or in the antigen sequence.

Learn what antibody epitopes are and explore key methods for antibody epitope mapping in drug development, diagnostics, vaccine design, and structural immunology.

Epitope mapping is defined as the process used to recognize and characterize the epitopes, or antigenic determinants, on an antigen that interact with antibodies or other molecules of interest.

What is Epitope Mapping? Epitope mapping is a pioneering technology that allows for the identification of the structure and sequence of an epitope. This is the basis of therapeutic antibody and diagnostic …

The six epitope mapping technologies used here can be divided into two groups. HDX, HRF, and XL are structure-based methods while ALN, Peptide Array, and DomX are function (binding) based methods.

May 18, 2023 · In this review, we describe in detail the principles of hydrogen deuterium exchange mass spectrometry in application to epitope mapping. We also show that a combination of MS-based …

Jun 24, 2025 · Epitope mapping is a crucial technique in immunology that involves identifying the specific regions on an antigen that are recognized by the immune system, particularly by antibodies, …

Sep 25, 2023 · Here, we briefly introduce the principles, advantages, and disadvantages of the latest epitope mapping techniques with examples for viral antigen discovery.