In addition to this, the chapter also features information on type of service (s) offered (gene synthesis, custom cloning, sub-cloning and others), cloning method (s) used (Restriction Enzyme Based ...

Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered. Restriction ...

Improvements to sequence- and ligation-independent cloning (SLIC) greatly increase its efficiency for difficult inserts. Sequence- and ligation-independent cloning (SLIC) is a seamless cloning method ...

Here are steps you can take to increase the odds of generating correct clones. PCR amplicons are commonly used for cloning. Primers made from synthetic oligonucleotides often contain a small ...

Traditional cloning and sequencing methods can be laborious, expensive, and time-consuming techniques, especially when applied to large sample numbers. Even for the routine cloning of small sample ...